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Enass Ali Alkhader

METHOD DEVELOPMENT AND VALIDATION OF
BTIPIVACAINE IN HT]MAN PLASMA AND THE
FEASABILITY TO BE APPLIED ON PREGNANT WOMEN
DURING LABOR
By
Enass Ali Alkhader
Supervisor
Prof. Tawfiq Arafat
Co-Supervisor
Dr. Wael Abu Dawih
A Thesis Submitted in Partial Fulfillment of the Requirements for the
Degree of Master of Science in Pharmaceutical Sciences at
Universitv of Petra
Faculty of Pharmacy and Medical Sciences
Amman-Jordan
June 2013
Method Development and Validation of Bupivacaine in
Human Plasma and the Feasibility to be Applied on Pregnant
Women During Labor
By
Enass Ali Al-khader
A Thesis Submitted in Partial Fulfillment of the Requirements for the
Degree of Master of Science in Pharmaceutical Sciences at University of
Petra
Faculty of Pharmacy and Medical Sciences
Amman-Jordan
June 2013
DEDICATIONS
First and foremost, I dedicate my work to Allah, the Almighty, lor he empowered
me the strength to persist all the way long, and granted me the blessing to finish
this thesis the wav it is.
To you my father; making you proud has always been my first aim, you have always
been my source of encouragement, support and inspiration throughout my whole life,
thank you for teaching me the impossible is nothing, with you by my side, I can reach
the stars.
To my beloved mother; you lend me a hand to stand up when everything seemed
like falling apart, without your full heart prayers I would have never achieved any
ofthis.
To my dear husband, Emad, thank you for your understanding and encouragement in
my moments ofcrisis, I would happily consider rny success as yours.
My brothers, sisters and friends deserve my wholehearted thanks as well.
lll
AKNOWLEDGMENTS
First of all, I would like to express my sincere appreciation to my supervisor Prol
Tawfiq Arafat for the continuous support in all stages of this thesis. His knowledge,
expertise, patience and guidance helped me to keep moving ahead with my work.
From the formative stages ofthis thesis, to the final draft, I owe an immense debt of
gratitude to my co-supervisor Dr. Wael Abu Dayyih, his assistance, advice and
spiritual and technical support enabled me to complete my work successfully and I
can't thank him enough.
I would like also to express my thanks and respect to Dr. Eyad Al-Mallah, Dr. Faisal
Al-Akayleh and Dr. Kamal Sweidan for being part of my committee and giving this
thesis a chance of their valuable time and scientific experience.
Last but not Least, I would like to thank the staff in Jordan Center for Pharmaceutical
Research (JCPR) and all of the staff in Faculty of Pharmacy and Medical Sciences at
University ofPetra for offering all the possible help.