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Ala’a Mohammed Saleem

 EFFECT OF GLUCOSAMINE ON THE
AVAILABILITY OF DIACEREIN IN
HUMAN BODY
By
Ala’a Mohammed Saleem
A Thesis Submitted in
Partial Fulfilment of the
Requirements for the Degree of
Master of Science
in pharmaceutical Sciences
at
Petra University,
Faculty of Pharmacy and Medical Sciences
Amman - Jordan
June 2013
II
EFFECT OF GLUCOSAMINE ON THE AVAILABILITY
OF DIACEREIN IN HUMAN BODY
By
Ala’a Mohammed Saleem
A Thesis Submitted in Partial Fulfilment of the Requirements for the Degree of
Master of Science
in Pharmaceutical Sciences
at
Petra University
Faculty of Pharmacy and Medical Sciences
Amman - Jordan
June 2013
 
III
Abstract
EFFECT OF GLUCOSAMINE ON THE AVAILABILITY
OF DIACEREIN IN HUMAN BODY
By
Ala’a Mohammed Saleem
Petra University, June 2013
Under the Supervision of Prof. Tawfiq Arafat & Prof. Tawfiq Alhussainy
In this pilot study we examined the hypothesis that glucosamine increases the
bioavailability of administered drugs if taken concomitantly with glucosamine. The
mechanism by which this is achieved is not yet fully understood. Previous work in rat
animal model has confirmed an increase in paracetamol bioavailability if administered
concomitantly with glucosamine. To test this hypothesis, this study used 6 human
subjects as pilot study to test the effect of glucosamine on the selected drug diacerein.
The study design of the experiment was planned over 7 days period which entailed
administering on the first day a single oral dose of 50 mg diacerein followed on the
2nd until 6th day of the study period by administering 500 mg oral dose twice daily of
glucosamine then on the 7th day administration of a single oral dose of 50 mg
diacerein. Blood samples were withdrawn on the first day on pre-determined time
schedule and on the 7th day to test for diacerein bioavailability. Withdrawn samples
IV
were treated in accordance with the validated method of analysis and concentration of
rhein was determined using HPLC analysis with fluorescent detector. The results were
analyzed statistically which indicated insignificant change in diacerein bioavailability.
We have reached a conclusion that diacerein metabolic pathway is not affected by
glucosamine due to absence of metabolism via cytochrome P450 pathway in
diacerein.
 
VI
Dedication
This work is dedicated to the ultimate grace in my life, my beloved family.
My warm-hearted mother Samar; the source of unconditional love and my
best friend, my brilliant father Mohammed; the pillar of strength in my
life and voice in my head motivating me to be the best, my brother Haidar;
the beacon of guidance and support, and my sister Doha for her kindness
and constant encouragement. Without you all, my life is empty, may God
protect you.
To my friends for their constant help, prayers and cheer.
To my homeland, of which I am deprived.
VII
Acknowledgement
I am profoundly touched by the blessings bestowed upon me for which I am eternally
grateful and in debt. “There is no good in life except gaining knowledge.”
In realization of this work I would like to thank my supervisor Prof. T. Arafat for his
direct and indirect support and assistance throughout my master degree without which
I would not have completed my work in the planned framework. I extend my thanks
to Prof. T. Alhussainy who co-supervised this work and lend great support in reading
and directing my thesis. Further, I would like to thank Prof. N. Idkaidek for the
assistance he has given me in the statistical analysis used in this work. Also, I would
like to thank Hamza and Munther from Jordan Center for Pharmaceutical Research
(JCPR) for their practical assistance.
I am deeply grateful to Science-forum for Research and Consultancies (SIPS) for the
constant support and encouragement especially Dr. Mohammed Saleem who is my
mentor and idol. Also, to my colleagues for their prayers and motivation.
At last but not least, for TRB Chemedica of Switzerland for allowing me the benefit
of knowledge into diacerein and the material support provided.