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Ala’a Mohammed Saleem

 EFFECT OF GLUCOSAMINE ON THE
AVAILABILITY OF DIACEREIN IN
HUMAN BODY
By
Ala’a Mohammed Saleem
A Thesis Submitted in
Partial Fulfilment of the
Requirements for the Degree of
Master of Science
in pharmaceutical Sciences
at
Petra University,
Faculty of Pharmacy and Medical Sciences
Amman - Jordan
June 2013
II
EFFECT OF GLUCOSAMINE ON THE AVAILABILITY
OF DIACEREIN IN HUMAN BODY
By
Ala’a Mohammed Saleem
A Thesis Submitted in Partial Fulfilment of the Requirements for the Degree of
Master of Science
in Pharmaceutical Sciences
at
Petra University
Faculty of Pharmacy and Medical Sciences
Amman - Jordan
June 2013
 
III
Abstract
EFFECT OF GLUCOSAMINE ON THE AVAILABILITY
OF DIACEREIN IN HUMAN BODY
By
Ala’a Mohammed Saleem
Petra University, June 2013
Under the Supervision of Prof. Tawfiq Arafat & Prof. Tawfiq Alhussainy
In this pilot study, we examined the hypothesis that glucosamine increases the bioavailability of administered drugs if taken concomitantly with glucosamine. The mechanism by which this is achieved is not yet fully understood. Previous work in rat animal model has confirmed an increase in paracetamol bioavailability if administered concomitantly with glucosamine. To test this hypothesis, this study used 6 human subjects as pilot study to test the effect of glucosamine on the selected drug diacerein. The study design of the experiment was planned over 7 days period which entailed administering on the first day a single oral dose of 50 mg diacerein followed on the 2nd until 6th day of the study period by administering 500 mg oral dose twice daily of glucosamine then on the 7th day administration of a single oral dose of 50 mg diacerein. Blood samples were withdrawn on the first day on pre-determined time schedule and on the 7th day to test for diacerein bioavailability. Withdrawn samples.
 
IV
were treated in accordance with the validated method of analysis and concentration of
rhein was determined using HPLC analysis with fluorescent detector. The results were
analyzed statistically which indicated insignificant change in diacerein bioavailability.
We have reached a conclusion that diacerein metabolic pathway is not affected by
glucosamine due to absence of metabolism via cytochrome P450 pathway in
diacerein. 
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Dedication
This work is dedicated to the ultimate grace in my life, my beloved family.
My warm-hearted mother Samar; the source of unconditional love and my
best friend, my brilliant father Mohammed; the pillar of strength in my
life and voice in my head motivating me to be the best, my brother Haidar;
the beacon of guidance and support, and my sister Doha for her kindness
and constant encouragement. Without you all, my life is empty, may God
protect you.
To my friends for their constant help, prayers and cheer.
To my homeland, of which I am deprived.