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Ahmed Issam Al-Kawaz

Validation and Determination of Candesartan with Different Juices in Rat Plasma
by using High Performance Liquid Chromatography/Mass Spectrometry
(HPLC/MS/MS).
By
Ahmed Issam Al-Kawaz
Supervisor:
Prof. Tawfiq Arafat
Co-Supervisor:
Dr. Wael Abu Dayyih
A Thesis Submitted in Partial Fulfillment of the Requirements for the Degree of
Master of Science in Pharmaceutical Sciences at
University of Petra
Faculty of Pharmacy and Medical Sciences
Amman-Jordan
January 2014
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Validation and Determination of Candesartan with Different Juices in Rat Plasma
by using High Performance Liquid Chromatography/Mass Spectrometry
(HPLC/MS/MS).
By
Ahmed Issam Al-Kawaz
A Thesis Submitted in Partial Fulfillment of the Requirements for the
Degree of Master of Science in Pharmaceutical Science at
University of Petra
Faculty of Pharmacy and Medical Sciences
Amman-Jordan
January 2014
 
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ABSTRACT
Validation and Determination of Candesartan with Different Juices in Rat Plasma
by using High Performance Liquid Chromatography/Mass Spectrometry
(HPLC/MS/MS).
By
Ahmed Issam Al-Kawaz
University of Petra, 2014
Supervisor Co-supervisor
Prof. Tawfiq Arafat Dr. Wael Abu Dayyih
A new validated simple, rapid and sensitive method for determination of candesartan in the presence of each juice has been applied by using High Performance Liquid Chromatography–Mass Spectrometry (HPLC/MS). The mobile phase was composed of (methanol, of 0.2% FA in water) was used as a mobile phase, ACE 5 C18 Column (50 X 2.1 mm), 5μ, and a flow rate of 1.0 ml/min were used, the autosampler injection volume was 5 microliters, and Irbesartan was used as internal standard, The precision of predicted measurements for candesartan was high (mean CV% <10%). The accuracy for candesartan over all the three days of validation and all the four tested target concentration was within the accepted criteria. The standard curves for candesartan matched the requirements, linear relation (R2) ranged between (0.996 to 1). According to the result obtained, the Cmax for candesartan alone was (964.692 ng/ml), there was no significant effect (P>0.05) of orange juice on candesartan Cmax (1253.163ng/ml). and for Licorice, the Cmax was (818.2868 ng/ml) which is also considered as a non- significant effect (P>0.05). on the other hand pomegranate shows to decrease the Cmax of candesartan (475.9673 ng/ml) which is a significant effect (P<0.05). candesartan plasma level was lowered to the half when combined with pomegranate, and almost at the same level when combined with both of orange and liquorice.
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I would especially like to thank my dear friends, Teeba Emad, Rafal Ammar, Ahmed
Basim, Rafif Raad at University of Petra. All of you have been there to support me in
writing, and incented me to strive towards my goal.
Special thanks to my M.S.c friends (Abdullah Nabil, Nada Ali, Ibrahem Adil, Nibras
Jamal, Noor Maan, Ragheed Adil, Mujtaba, Zena Hilal and Zainab Al Obaidy).
Also I want to thank all my friends who have been very supportive. (Taha alKhanchi,
Maashar, Usama Mezil, Bashar Younis, Ban Thiab, Shahad Faisal, Ahmed Al-Azzawi,
Vegen, Mohannad Kattan, Hesham, Khalid Al-Haidari, Riham Nasir, Mais Jamal)
In the end, my thanks go to for my beloved brothers, my extended family, especially my
aunt Anaam. And I would love to dedicate this work, to my Father Dr.Issam and my
mother Dr,Intisar whom I know their prayers and love still protecting and guiding me.